This was the second model year for the RX 300, Lexus's car-based luxury SUV. By 2000, the RX 300 accounted for one half of all Lexus sold in the United States. Drivers were pleased that many of the negatives often attributed to SUVs and trucks were absent from the RX. Then again, so were many of the benefits.

Despite the addition of a new exterior color, the RX remains unchanged from 1999. The 3.0 Liter V-6 provides 220 hp and 222 lb.-ft. of torque. Gas mileage was 19/24 for the front wheel model and 18/22 for the AWD. The RX seats five and offers as much additional storage space as a Jeep Grand Cherokee. Since Lexus estimated that just 7% of RX buyers will venture off-road, the full time AWD continued to be optional. Ground clearance was bumped up 1/5 of an inch.

RX reviews for 2000 were a mixed bag. Some loved what others hated. The most common rave was the smooth and quiet ride that Lexus is known for. The RX's unibody construction provides a cushioned ride which was quite unexpected from an SUV. Frequent complaints were unfriendly ergonomics, including an awkward display screen disproportionately large for its functionality. The discovery that the RX is much more of a car was upsetting to many drivers expecting an SUV.